Isolation and screening of thermophilic and psychotrophs bacterial strains used in the composting process

Psychrotrophs and thermophilic are two categories of microbes who can survive in extreme low and high temperature environments. Theoretically, the introduction of such micro-organisms can increase the initial temperature at the early mesophilic phase or maintain the high temperature at the thermophilic phase of aerobic composting. And the key is to find the strains growing fast and decomposing efficiently of protein, starch, cellulose and other organic substances in different temperature environments.

Further authors:
L. Y. Wang - Department of Ecology and Ecological Engineering, China
D. M. Wang - Department of Ecology and Ecological Engineering, China

10 samples from different organic sources, as sewage water, soil and animal manure were collected in Heilongjiang province, China, and from which we isolated four psychrotrophs bacterial strains, as B6-38,B9-13,B10-40 and B10- 44. They all showed the characteristics of fast growing and rapid degrading of organic substances at low temperature (15 ℃). Determined with 16S rDNA gene sequencing and growth characteristics, B6-38 and B10-40 are associated with the genera Aeromonasa, B9-13 with the genera Enterobacter and B10-44 with the genera Paenibacillus. A composting experiment with chicken manure and sawdust was carried out in a small-sized container to study the effects of microbial inoculation(B6-38,B9-13,B10-40 and B10-44, individual strain)on the composting process. The results showed that the rate of temperature growth of the treatment with B6-38 was the highest, 61.79% higher than control, among those 4 treatments. Meanwhile, two cellulose decomposing thermophilic bacterial strains, HNS39 and HNC41, were screened from 13 thermoplic compost samples (at high temperature phase, about 80oC) from Hebei, Hainan and Shandong provinces, China. Both are associated with the genera Geobacillus determined by using 16S rDNA gene sequencing and growth characteristics. The similar composting experiment was done to study the effects of microbial inoculation(HNS39, HNC41 and the mixture). The results showed that the use of the HNS39 and HNC41 were more effective in terms of the increasing of initial temperature, maintaining of high temperature at thermophilic phase and slowing down of the temperature decline at maturation phase. However, there was no significant difference between the effect of individual strain and their mixture (1:1 v/v).

Copyright: © European Compost Network ECN e.V.
Quelle: Orbit 2012 (Juni 2012)
Seiten: 8
Preis inkl. MwSt.: € 8,00
Autor: Prof. Dr. Ji Li

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